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Colorimetric determination of sterols in vegetable oils with chloramine T and sulfuric acid reagent
Author(s) -
Ahuja K. L.,
Bajaj K. L.
Publication year - 1980
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02668253
Subject(s) - unsaponifiable , reagent , chemistry , saponification , chromatography , petroleum ether , sulfuric acid , thin layer chromatography , silica gel , chloramine t , ether , organic chemistry , extraction (chemistry)
A new colorimetric method for determination of sterols in vegetable oils using chloramine T and conc sulfuric acid reagent is described. After saponification of oils, the sterols present in the unsaponifiable matter are separated by thin layer chromatography (TLC) on Silica Gel G plates in the solvent system ethyl ether/petroleum ether (40–60 C) (1:1). The sterols are treated with 1% chloramine T in cone H 2 SO 4 at 100 C for 12 min to develop the yellowish‐brown color which is read at 400 nm. Sterol content is calculated against a cholesterol standard. The method is at least 5 times more sensitive than that which uses Liebermann‐Burchard reagent; color formed is stable for 48 hr.