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Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography
Author(s) -
Rouser George,
Galli Claudio,
Lieber Ellen,
Blank M. L.,
Privett O. S.
Publication year - 1964
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02663969
Subject(s) - chromatography , chemistry , column chromatography , thin layer chromatography , fractionation , cellulose , chromatography column , two dimensional chromatography , densitometry , quantitative analysis (chemistry) , high performance thin layer chromatography , gas chromatography , organic chemistry , physics , quantum mechanics
A quantitative chromatographic procedure for the fractionation of complex lipid mixtures is described. The method utilizes diethylaminoethyl (DEAE) cellulose column chromatography followed by thin layer chromatography (TLC). Spots produced in TLC are charred with sulfuric acid‐potassium dichromate and heat and are then measured by quantitative densitometry. Results obtained with beef brain and beef heart mitochondrial lipids are presented, and the close correspondence between column isolation procedures and the new procedure is demonstrated. Methods utilizing only column chromatography, column chromatography and TLC, and one‐ and two‐dimensional TLC without column chromatography are compared.