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Number of double bonds in fatty acids from fats and oils by hplc using pentafluorobenzyl esters
Author(s) -
Netting A. G.
Publication year - 1986
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02663952
Subject(s) - degree of unsaturation , saponification , chemistry , chromatography , double bond , bromide , organic chemistry , high performance liquid chromatography , fatty acid , polyunsaturated fatty acid , chromatographic separation
A simple, one‐pot procedure for the saponification of fats and oils and the subsequent esterification of the resulting fatty acid salts with pentafluorobenzyl bromide is presented. A normal phase high pressure liquid Chromatographic procedure for the separation of these pentafluorobenzyl esters, primarily on the degree of unsaturation, is also given. Chromatographic detection of the esters can be carried out conveniently at 254 nm, and the procedure should therefore find application in the routine determination of the number of double bonds in fatty acids from fats and oils.