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Metal salts requisite for the production of eicosapentaenoic acid by a marine bacterium isolated from mackerel intestines
Author(s) -
Akimoto Masamichi,
Ishii Tetsuya,
Yamagaki Koji,
Ohtaguchi Kazuhisa,
Koide Kozo,
Yazawa Kazunaga
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02663822
Subject(s) - sodium , potassium , magnesium , eicosapentaenoic acid , chemistry , food science , yield (engineering) , mackerel , bacteria , chloride , calcium , yeast extract , metal , nuclear chemistry , biochemistry , biology , polyunsaturated fatty acid , fermentation , fatty acid , organic chemistry , fishery , materials science , fish <actinopterygii> , genetics , metallurgy
Metal salts important for the growth and 5,8,11,14,17‐ ciseicosapentaenoic acid EPA) production of a bacterium isolated from Pacific mackerel intestines were investigated at 25°C in culture media containing 1.0% peptone and 0.50% yeast extract, and the composition of an optimum culture medium was determined. This bacterium could grow in the media in which sodium chloride was the sole added inorganic component. By raising the concentration of sodium chloride from 1.2 to 2.4%, the yield of bacterial cells increased and the yield of EPA reached a maximum at 2.0% NaCl concentration. In contrast to calcium chloride, potassium chloride and magnesium chloride as second metal salts promoted the growth of this bacterium at relatively low concentrations without inhibiting the accumulation of EPA. The yield of EPA reached its maximum value of 51.9 mg/L of culture broth at 8 hr at 2.0% NaCl, 0.15% KCl and 0.16% MgCl 2 concentrations. This yield of EPA was 20% greater than that obtained with Jamarin S artificial sea water.