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Measurement of oxidative interactions of cholesterol
Author(s) -
Nawar W. W.,
Kim S. K.,
Li Y. J.,
Vajdi M.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02663820
Subject(s) - chemistry , oxidative phosphorylation , cholesterol , cysteine , amino acid , capillary gas chromatography , silicic acid , gas chromatography , alanine , chromatography , biochemistry , organic chemistry , enzyme
A simple analytical technique, using a silicic acid minicolumn and capillary gas chromatography, was used for measuring the oxidative interactions of cholesterol with other compounds. When triacylglycerols were added to cholesterol before heating at 180°C, the latter oxidized faster than cholesterol heated alone, and a relatively high amount of epoxides was found. Dipahnitoylphosphatidylethanolamine and all the amino acids tested showed a protective effect, with cysteine and alanine being the most effective. The results of this study indicate that the varius compounds added not only influenced the rate of cholesterol oxidation, but also exerted different influences on its oxidative pathway.