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Determination of chlorophyll in ground rapeseed using a modified near infrared reflectance spectrophotometer
Author(s) -
Tkachuk R.,
Mellish V. J.,
Daun J. K.,
Macri L. J.
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02663082
Subject(s) - rapeseed , calibration , wavelength , chlorophyll , correlation coefficient , linear regression , remote sensing , infrared , near infrared spectroscopy , analytical chemistry (journal) , chlorophyll a , coefficient of determination , chemistry , mathematics , environmental science , materials science , optics , statistics , physics , chromatography , optoelectronics , geography , food science , biochemistry , organic chemistry
A rapid and accurate method has been developed for the analysis of chlorophyll in ground rapeseed using a modified near infrared filter instrument. Ground rapeseed was scanned with a Cary 17 spectrophotometer, and optimal wavelengths for chlorophyll prediction (674, 696 nm) were selected from the visible wavelength region by multiple linear regression analysis. Six Dickey‐john Instalab 600's were modified to analyze chlorophyll in ground rapeseed by replacing two standard near infrared filters with two filters having central wavelengths of 674 and 696 nm. Calibration equations incorporating data from three wavelengths (674, 696 and 2100 nm) had an average multiple correlation coefficient (R) of 0.980 and standard error of estimate (SEE) of 3.1 ppm. Instrument prediction of chlorophyll agreed well with reference solvent extraction analyses (standard error of prediction, SEP=3.0 ppm). A universal calibration equation developed for the determination of chlorophyll in ground rapeseed was tested with several modified instruments and performed well.