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A novel compound, 7,10‐dihydroxy‐8(E)‐octadecenoic acid from oleic acid by bioconversion
Author(s) -
Hou C. T.,
Bagby M. O.,
Plattner R. D.,
Koritala S.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02662326
Subject(s) - bioconversion , oleic acid , hydroxylation , yield (engineering) , substrate (aquarium) , chemistry , double bond , organic chemistry , chromatography , food science , biochemistry , biology , enzyme , fermentation , materials science , ecology , metallurgy
Sixty‐two cultures from the Agricultural Research Service (ARS) Culture Collection and 10 cultures isolated from soil and water samples in Illinois were screened for their ability to convert agricultural oils to value‐added industrial chemicals. A new compound, 7,10‐dihydroxy‐8(E)‐octadecenoic acid (DOD), was produced from oleic acid at a yield of greater than 60% by bacterial strain PR3 which was isolated from a water sample in Morton, IL. To our knowledge, DOD has not been previously reported. The optimum time, pH and temperature for the production of DOD were 2 days, 7.0, and 30°C, respectively. The production of DOD is unique in that it involves hydroxylation at two positions and rearrangement of the double bond of the substrate molecule.