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Use of aqueous HCI/MeOH as esterification reagent for analysis of fatty acids derived from soybean lipids
Author(s) -
Jham G. N.,
Teles F. F. F.,
Campos L. G.
Publication year - 1982
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02662261
Subject(s) - reagent , chemistry , petroleum ether , hydrolysis , chromatography , aqueous solution , soybean oil , extraction (chemistry) , ether , organic chemistry , biochemistry
Abstract A quick, reliable and very inexpensive method is described for the analysis of fatty acids derived from soybean lipids. The method involves extraction of soybean lipids with petroleum ether, followed by hydrolysis of lipids with KOH/MeOH (0.5 M) for 5 min at 100 C followed by esterification with aq. HC1 (36%)/MeOH (4:1, v/v) for 15 min at 100 C. No problems were encountered with the esterifica‐tion procedure in the presence of water and the procedure gave results comparable to die more conventional BF 3 /MeOH reagent. The aq. HCI/MeOH reagent is several hundred times cheaper than BF 3 /MeOH, and does not compromise the efficiency of the reagent.