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High performance liquid chromatography of phospholipids with flame ionization detection
Author(s) -
Grieser Merlin D.,
Geske Jerome N.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02661977
Subject(s) - ammonium hydroxide , chromatography , chemistry , lecithin , resolution (logic) , flame ionization detector , high performance liquid chromatography , chloroform , methanol , solvent , hydroxide , analytical chemistry (journal) , gas chromatography , organic chemistry , artificial intelligence , computer science
The three major soy phospholipids were determined by normal phase high performance liquid chromatography with flame ionization detection. A Beckman Ultrasphere silica column was utilized with a solvent gradient composed of chloroform and premixed methanol/ ammonium hydroxide. This chromatographic system produced excellent resolution of the three major phospholipids and triglyceride component as well as some of the minor constituents in soy oil lecithin. Ammonium hydroxide improved peak shape and resolution but caused the peaks for the phospholipids to appear as doublets. Quantitative analysis was accomplished by the external standard method.