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Automated quantitative analysis of isolated (nonconjugated) trans isomers using fourier transform infrared spectroscopy incorporating improvements in the procedure
Author(s) -
Sleeter Ronald T.,
Matlock Mark G.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02661799
Subject(s) - fourier transform infrared spectroscopy , fourier transform , analytical chemistry (journal) , cis–trans isomerism , infrared , content (measure theory) , spectroscopy , correlation coefficient , near infrared spectroscopy , fourier transform spectroscopy , infrared spectroscopy , dilution , chemistry , materials science , chromatography , mathematics , optics , statistics , organic chemistry , physics , mathematical analysis , quantum mechanics , thermodynamics
A method for determining the isolated (nonconjugated) trans isomer content of fats and oils was developed using Fourier Transform Infrared Spectroscopy (FTIR), which permits automatic sample analysis and calculation of percent trans content. Integrated band areas were used as a measure of the intensity of the band associated with the trans C=C double bond. Measured band areas for samples with known percent trans content were fit with a second order polynomial, resulting in a correlation coefficient of 0.99998 and a standard error of estimate of 0.11 over the range of 0 to 50% trans content. This technique also allows the analysis of neat samples, a significant improvement over current procedures in that it eliminates the need for dilution by volatile solvents and any attendant errors.