Premium
Quantitative Determination of phosphatidylcholine by an HPLC‐RI system
Author(s) -
Yamagishi Toshihiko,
Akiyama Hiroshi,
Kimura Shinichi,
Toyoda Masashi
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02660751
Subject(s) - high performance liquid chromatography , chromatography , phosphatidylcholine , chemistry , quantitative analysis (chemistry) , hexane , phospholipid , biochemistry , membrane
The following describes the quantitative determining method for phosphatidylcholine (PC) using the HPLC‐RI system which we have developed. It uses Lichromsorb, Si 60 (10 μm), 4.6 mm × 250 mm as the column and a mobile phase consisting of n‐hexane/isopropanol/water =1:4:1. In this report, we compared data from selected high‐purity (60–100 wt%) samples using the HPLC‐RI, HPLC‐UV and conventional TLC‐P methods. Under the conditions we described, the HPLC‐UV method was somewhat affected by fatty acid compositions. As a result, there were some inconsistencies in the measured values. However, the HPLC‐RI method we propose was applicable to PC from both egg yolk and soybeans. In addition, the HPLC‐RI method produced data which correlated well with data from the TLC‐P method, and this data was highly accurate and exhibited satisfac‐tory reproductibility.