Premium
A simple and cost‐effective gram‐scale chromatographic method for the purification of soybean phospholipids
Author(s) -
De Meulenaer B.,
Van der Meeren P.,
Vanderdeelen J.,
Baert L.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02660725
Subject(s) - chromatography , chemistry , fractionation , elution , solvent , lecithin , hexane , silica gel , gram , phase (matter) , soybean oil , organic chemistry , bacteria , biochemistry , biology , genetics
A method was developed for the preparative fractionation of soybean lecithin to enable the study of the functional properties of pure soybean phospholipids. Hereby, a coarse and irregularly shaped silica gel was used as the stationary phase, whereas the mobile phase consisted of three mixtures of hexane, 2‐propanol, and water with increasing polarity. These solvents were included in a step gradient, which was formed by an isocratic pump connected to a solvent switcher. With this system, two grams of soybean lecithin were fractionated. The purity was evaluated by analytical high‐performance liquid chromatography, and the recovery was estimated from concentration determinations by flow injection analysis. From these results, it was concluded that 60 to 75% of the three major soybean phospholipids could be recovered with a purity of at least 93%. Only 1.5 L of solvents were needed for both the column equilibration and the elution of all soybean phospholipids.