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High performance liquid chromatography of oxygenated cholesterols and related compounds
Author(s) -
Tsai LeeShin,
Hudson C. A.
Publication year - 1981
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02659661
Subject(s) - chemistry , side chain , silicic acid , hexane , tetrahydrofuran , ring (chemistry) , chromatography , gas chromatography , organic chemistry , solvent , polymer
Twenty‐four oxygenated cholesterols and structurally related compounds were analyzed by high performance liquid chromatography with silicic acid column and various mobile phases. Hexane–propanol was superior to hexane/tetrahydrofuran and hexane/ethyl acetate for the separation of oxygenated cholesterols. The retention volumes of oxygenated cholesterols depended on the characteristics of the substituting group, its position of substitution, as well as its orientation. The effect of various functional groups at different positions on cholesterol molecules, in general order of decreasing retention volumes, were: hydroxy on the ring, carbonyl on the ring, epoxy on the ring, hydroxy on the side chain, and carbonyl on the side chain. Synergistic effect of multiple hydroxyl substitutions on cholesterol was observed.

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