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Silver‐complexation liquid chromatography for fast, high‐resolution separations of triacylglycerols
Author(s) -
Jeffrey B. S. J.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02657678
Subject(s) - chromatography , elution , chemistry , resolution (logic) , reproducibility , solvent , high resolution , gas chromatography , gradient elution , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , remote sensing , artificial intelligence , computer science , geology
A high‐speed, high‐resolution approach to silvercomplexation chromatography of triacylglycerols in vegetable fats and cocoa butter equivalents is described. Trisaturated and major isomeric unsaturated species with up to five double bonds are eluted within 12 min. A simple change of solvent composition enables elution of species with up to nine double bonds within the same time‐frame. Throughput is three samples/hr (70 samples/24 hr with an automatic system). Short‐term reproducibility of analytical data for the system gives standard deviations similar to those obtained in practice for high‐resolution capillary gas chromatography of triacylglycerols, and quantitative accuracy is good. Details of column preparation, solvent gradient and system operation are given.