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Separation of fatty acid esters from cholesterol in esterified natural and synthetic mixtures by supercritical carbon dioxide
Author(s) -
Yeh AnI,
Liang J. H.,
Hwang L. S.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02657613
Subject(s) - supercritical carbon dioxide , solubility , chemistry , carbon dioxide , supercritical fluid , squid , chromatography , fatty acid , cholesterol , organic chemistry , biochemistry , ecology , biology
The solubility of cholesterol in supercritical carbon dioxide was determined by a continuous flow method. The solubility of cholesterol increased with increasing pressure and exhibited retrograde behavior. The Chrastil equation was used to describe the relationship between solubility and the density of carbon dioxide. A model mixture was made by adding cholesterol and fatty acid esters together. Squid visceral oil was esterified as the feed material. Both the model mixture and esterified squid visceral oil were extracted by supercritical carbon dioxide. The experimental results showed that cholesterol could be removed from a model mixture and from esterified squid visceral oil at low pressure (1500 psig) and high temperature (328.2°K). Under these conditions, cholesterol content in the extract was reduced from 2867 mg/100 g to 14.1 mg/100 g.