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Combination of silver ion and reversed‐phase high‐performance liquid chromatography in the fractionation of herring oil triacylglycerols
Author(s) -
Laaksoo Päivi,
Christie William W.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02657612
Subject(s) - degree of unsaturation , high performance liquid chromatography , chromatography , fractionation , chemistry , clupea , herring , double bond , fatty acid , organic chemistry , fish <actinopterygii> , biology , fishery
Triacylglycerols from North Atlantic herring (Clupea harengus) were separated according to the degree of unsaturation by high performance liquid chromatography (HPLC) in the silver ion mode. Each of the eleven fractions collected was then separated by reversed‐ phase HPLC, which in these circumstances separated the molecules according to the combined chain‐ lengths of the fatty acyl residues only. One hundred thirty fractions were obtained for fatty acid analysis. Almost 50% of the triacylglycerol molecules had six or more double bonds in their fatty acyl residues. Saturated‐dimonoenes and disaturated‐ monoenes, 18.9% and 10.4%, respectively, were the most plentiful fractions of the more saturated species. Such a complex mixture of molecules was present that the most abundant subfractions from reversed‐ phase HPLC represented less than 5% of the total. Indeed, the largest single molecular species [16:0‐ 22:l‐ 22:6(n− 3)] represented only 2.8% of the total. These sequential analyses by complementary techniques made it possible to obtain a considerable amount of information on the composition of molecular species, but it was still not possible to identify all components.