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Analysis of neutral lipids and glycerolysis products from olive oil by liquid chromatography
Author(s) -
Yang Baokang,
Chen Jyhping
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02657548
Subject(s) - chromatography , chemistry , chloroform , acetone , formic acid , glyceride , chromatography detector , elution , olive oil , silica gel , high performance liquid chromatography , fatty acid , organic chemistry , food science
A simple method was developed to separate model triglycerides, 1,3‐diglycerides, 1,2‐diglycerides, monoglycerides and free fatty acids and products generated in enzymatic glycerolysis of olive oil by high‐performance liquid chromatography with a laser light‐scattering detector. The separation was carried out with a silica column at 30°C. A gradient elution with mobile phase A (hex‐ane/chloroform/formic acid) and mobile phase B (hex‐ane/acetone/chloroform) can separate the compounds into distinct peaks in less than 20 min.
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