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Antioxidative activity measurement in lipid peroxidation systems with malonaldehyde and 4‐hydroxy nonenal
Author(s) -
Tamura H.,
Shibamoto T.
Publication year - 1991
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02657540
Subject(s) - chemistry , lipid peroxidation , microsome , antioxidant , vitamin e , ferulic acid , nuclear chemistry , vitamin , ethylenediamine , biochemistry , chromatography , organic chemistry , enzyme
Antioxidative activities of vitamin E, ethylenediamine tetraacetic acid (EDTA), ferulic acid, and butylated hydroxy toluene (BHT) were monitored with lipid peroxidation systems. Formation of malonaldehyde (MA) and 4‐hydroxy nonenal (4‐HN) from ethyl linoleate or rat liver microsome oxidized by FeCl 2 /H 2 O 2 or ADP/FeSO 4 was measured by gas chromatography. Over 90% of MA and 4‐HN formation was inhibited by 100 mmol/L of vitamin E. A synthetic antioxidant, BHT, showed the strongest antioxidative activity, followed by that of vitamin E, whereas EDTA accelerated formation of MA and 4‐HN in the ethyl linoleate/FeCl 2 /H 2 O 2 system. Vitamin E did not suppress lipid peroxidation significantly when microsome was oxidized by ADP/FeSO 4 . EDTA inhibited oxidation of microsome by ADP/FeSO 4 considerably; in contrast, it did not in the case of oxidation by FeCl 2 /H 2 O 2 .