Premium
Gas‐liquid chromatography of polar fatty derivatives
Author(s) -
Morrissette R. A.,
Link W. E.
Publication year - 1964
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02654872
Subject(s) - triacetin , polar , chemistry , fatty acid , polyamide , amide , substrate (aquarium) , organic chemistry , conjugated system , chemical polarity , gas chromatography , resolution (logic) , chromatography , carbon chain , glycerol , molecule , polymer , physics , oceanography , astronomy , artificial intelligence , computer science , geology
The direct separation of fatty amides is achieved using a polyamide. Versamid 900, as the partitioning agent on a support which need not be previously impregnated with strong alkali or acid, procedures usually followed in the GLC separation of highly polar materials. The combination of a neutral support and polar substrate permits the separation of unsubstituted and substituted long chain fatty amides with as many as 24 carbon atoms with good resolution, in a reasonable time, and with good peak symmetry. The observed area responses agree with the wt percentages of standard amide mixtures, indicating that no loss of amides occurs on the column under the conditions employed. The Versamid column has proved useful in the analysis of other polar fatty derivatives. Conjugated dienoic and trienoic acids run as their methyl esters are retarded sufficiently on Versamid 900 so that they may be estimated in the presence of other fatty acids. Mixtures of compounds with varying polarity, such as mono‐, diand triacetin, and glycerol, may be separated easily. Hydroxy and normal fatty acid methyl esters give equally summetrical peaks.