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Glass paper chromatography and elatography of triglycerides
Author(s) -
Swartwout J. R.,
Gross R. J.
Publication year - 1964
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02654819
Subject(s) - sodium methoxide , silica gel , chromatography , chemistry , solvent , pyridine , fatty acid , fatty acid methyl ester , organic chemistry , methanol , catalysis , biodiesel
A degree of reverse phase separation of triglycerides can be obtained with ascending chromatography on uncoated glass paper using a pyridine: water solvent system. The same R f values result when silica gel coated paper is used. To extend the usefulness of this separation, the glyceryl esters are converted to methyl esters in situ using sodium methoxide. This permits, on silica gel coated paper, glyceryl ester separation in one dimension, eslatography to the methyl esters, and methyl ester separation in the second dimension. In mixtures of lipids such as serum extracts, the lipid groups may be separated in one dimension and the fatty acid methyl esters in the second dimension.