Premium
Determination of free gossypol in cottonseed materials
Author(s) -
Pons Walter A.,
Guthrie John D.
Publication year - 1949
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02651512
Subject(s) - gossypol , cottonseed , acetone , extraction (chemistry) , chemistry , aqueous solution , filtration (mathematics) , chromatography , cottonseed oil , organic chemistry , biochemistry , mathematics , food science , statistics
Summary A method for the determination of free gossypol in cottonseed materials is described. The method consists of extraction of gossypol with a measured volume of 70% aqueous acetone on a shaker for one hour, filtration, and colorimetric analysis for gossypol in an aliquot of the filtrate by means of the reaction between gossypol and p ‐anisidine. The conditions for complete extraction of gossypol from various types of cottonseed materials have been investigated, the stability of gossypol in aqueous acetone has been demonstrated, and data are presented on recovery of gossypol added to cottonseed materials. The method has been compared with the method of Smith (2).