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Sesame Oil. IV. Determination of free and bound sesamol
Author(s) -
Budowski Pierre,
O'Connor R. T.,
Field E. T.
Publication year - 1950
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02649317
Subject(s) - sesamol , chemistry , sesame oil , aqueous solution , chromatography , octane , extraction (chemistry) , sulfuric acid , organic chemistry , antioxidant , sesamum , horticulture , biology
Summary The Villavecchia test was adapted for the quantitative determination of sesamol in sesame oil. Of the several aromatic aldehydes tested, furfural, in the presence of aqueous sulfuric acid (sp. gr. 1.37 at 15°C.), proved to be the most suitable for the quantitative measurement of the color produced with sesamol. The oil, dissolved in iso‐octane (10 g. per 100 ml.), is shaken with dilute aqueous‐alcoholic potassium hydroxide to remove the free, but not the bound sesamol. The modified Villavecchia test is applied to a) the original iso‐ octane solution of the oil, b) the iso‐octane solution after extraction with alkali, and/or c) the aqueous‐alcoholic extract. The optical densities, read at a wavelength of 518 mμ, correspond to the total bound, and free sesamol, respectively. The method was applied to the determination of free, bound, and total sesamol in a number of crude, refined, bleached, and deodorized sesame oils. Agreement between the values for total and free plus bound sesamol was close. Four crude sesame oils of different origins had total contents of sesamol ranging from 0.13 to 0.17%, of which nearly all was in the bound form. The yields of added sesamol varied from 95 to 106%.