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Lipids of the cottonseed. III. Perchloric acid ashing of lipids for the determination of phosphorus
Author(s) -
Frampton Ver L.,
Maseles Ruth Dodds,
Martin Garland Ned
Publication year - 1948
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02645898
Subject(s) - perchloric acid , cottonseed , ashing , chemistry , cottonseed oil , molybdenum blue , phosphorus , solvent , molybdenum , nitric acid , chromatography , inorganic chemistry , biochemistry , organic chemistry , food science , physics , quantum mechanics
SummaryCottonseed oil is effectively oxidized with a mixture of perchloric and nitric acids in connection with the determination of phosphorus by the Fisk and Subbarow method. The development of the molybdenum blue in the procedure is independent of the acid content in the reducing medium up to 0.5 normal. The presence of perchloric acid does not interfere with the development of the molybdenum blue. The phosphorus content of cottonseed extracts depends on the nature of the solvent. The quantities obtained varied from 0.0071 to 0.117%. A phospholipid containing 3.73% phosphorus was isolated from cottonseed.