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Permeability alterations in unilamellar liposomes due to betaine‐type zwitterionic and anionic surfactant mixed systems
Author(s) -
Maza A.,
Parra J. L.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02641004
Subject(s) - liposome , chemistry , pulmonary surfactant , partition coefficient , chromatography , phosphatidylcholine , micelle , sodium dodecyl sulfate , aqueous solution , critical micelle concentration , bilayer , betaine , phosphatidic acid , mole fraction , phospholipid , lipid bilayer , membrane , organic chemistry , biochemistry
The alterations caused by betaine‐type zwitterionic and anionic surfactant mixed systems in the permeability of unilamellar liposomes have been investigated. The partition coefficient of these systems, at different molar fractions, between the aqueous phase and the lipid bilayer of liposomes has been determined. These surfactant mixed systems were formed by N ‐dodecyl‐ N,N ‐dimethylbetaine (C 12 ‐Bet) and sodium dodecyl sulfate (SDS) in the presence of 20 mM PIPES buffer and 110 mM Na 2 SO 4 , at pH 7.21. Unilamellar liposomes were prepared from egg phosphatidylcholine and phosphatidic acid (9:1 molar ratio). The release of the fluorescent agent 5‐(6)‐carboxyfluorescein induced by the systems has been studied at sub‐solubilizing concentrations. When the molar fraction of C 12 ‐Bet/SDS is about 0.4, the critical micelle concentration values of these systems exhibit a minimum, whereas their partition coefficient between the aqueous phase and lipid bilayer of lipid bilayers shows a maximum. There is a consistent correlation between the partition coefficient and the ability of the different systems of surfactants to modify the permeability of liposomes.