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Chromatographic separation of unsaturated fatty acids as their 2,4‐dinitrobenzenesulfenyl chloride derivatives
Author(s) -
Simmons R. O.,
Quackenbush F. W.
Publication year - 1953
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02640978
Subject(s) - chemistry , chromatography , composition (language) , elution , linoleic acid , chloride , organic chemistry , absorption (acoustics) , oleic acid , chromatographic separation , fatty acid , high performance liquid chromatography , biochemistry , materials science , philosophy , linguistics , composite material
Summary Oleic, linoleic, and linolenic acids were found to react with 2,4‐dinitrobenzenesulfenyl chloride to form addition products of constant composition. Saturated acids did not react. The derivatives of mixed acids were separable into well defined bands on MgSO 4 columns and recoverable as individual compounds in the eluate, with yields above 95% in each case. This technique provided the basis for a new method of analysis, which we have called the “isolation” method. Results obtained in the analysis of 100‐mg. samples of common vegetable oils compared favorably with those obtained by the spectral absorption method except that isolation values for saturated acids were lower.