Isolation and identification of carbonyl compounds formed by autoxidation of ammonium linoleate

SummaryThe isolation and identification of carbonyl compounds formed by autoxidation of pure ammonium linoleate (0.02 M. in water) has been carried out. The autoxidation has been performed according to a method described by Tappel (23). A solution of 0.02 M. ammonium linoleate in 0.1 M. phosphate buffer of pH 6.9, containing 10 −4 M. CuSO 4 , was subjected to autoxidation at 37.2°C. The isolation and identification of the D.N.P.‐ones of the carbonyl compounds have been carried out, using a method of partition and adsorption chromatography published by van Duin (10, 11). It was demonstrated that the principal compounds formed were n‐hexanal, 2‐octenal, and 2,4‐decadienal. The formation of these aldehydes is fully in line with the theories of autoxidation of unsaturated fatty acids (1, 3, 13) and of the dismutation of the hydroperoxides formed (2).