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Determination of total gossypol pigments in cottonseed oils
Author(s) -
Pons Walter A.,
Hoffpauir Carroll L.,
O’Connor Robert T.
Publication year - 1951
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02639740
Subject(s) - gossypol , cottonseed , solvent , cottonseed oil , pigment , chemistry , reagent , chromatography , hexane , organic chemistry , food science , biochemistry
Summary A method for the determination of total gossypol pigments in cottonseed oils is described. The oil is dissolved in a hexane‐isopropanol solvent and gossypol pigments are determined in an aliquot of the solution by means of the color developed with p ‐anisidine. The effect of the solvent and the oil concentration on the color developed in the method has been investigated. The stability of gossypol in the solvent employed has been demonstrated and data are presented on the recovery of gossypol added to cottonseed oils. Spectrophotometric evidence is presented indicating that a number of gossypol‐like pigments are present in crude cottonseed oils and that all of these pigments react with p ‐anisidine to give reaction products spectrophotometrically identical with that obtained for pure gossypol with the same reagent.