Premium
Extraction of lipids from cottonseed tissue: V. Ultrastructural effects of extraction with hexane‐acetic acid
Author(s) -
Jacks T. J.,
Yatsu L. Y.,
Hensarling T. P.
Publication year - 1974
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02639731
Subject(s) - hexane , extraction (chemistry) , acetic acid , cytoplasm , cottonseed , chemistry , chromatography , electron microscope , cottonseed oil , lipid droplet , biochemistry , food science , physics , optics
Cottonseed tissue was extracted with acidified hexane (hexane containing 2–25% acetic acid, v/v) and then examined with an electron microscope. In all cases, the contents of the oil‐rich spherosomes were emptied and cytoplasm remained intact after lipid extraction. However, membranous elements of the cytoplasm appeared diffuse and disorganized. The possible effect of this disorganization of membranes in accounting for the greater amount of lipid extracted by acidified hexane than by hexane is discussed.