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Determination of free and conjugated triclosan in blood by electron capture gas liquid chromatography
Author(s) -
Schulze Janos,
Marquardt FritzHans,
Lyman Frank,
Spitzer Carol
Publication year - 1975
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02639144
Subject(s) - triclosan , chromatography , gas chromatography , chemistry , hydrochloric acid , conjugated system , hydrolysis , extraction (chemistry) , hexane , organic chemistry , medicine , pathology , polymer
Unconjugated triclosan in blood can be determined by extraction with hexane, followed by its conversion to an acetyl derivative and analysis by electron capture gas liquid chromatography. The conjugated triclosan can be analyzed by an identical procedure, after the complexes have been hydrolyzed with hydrochloric acid. The method was validated by recovery studies using spiking and radioactive techniques and can be used for the determination of triclosan in blood in the concentration range of 1 ppb (ng/ml) to 100 ppm (µg/ml). The precision and accuracy of the method are discussed, and examples of its application are presented.