Premium
Newer techniques in protein isolation and characterization
Author(s) -
Rothfus John A.
Publication year - 1970
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02638993
Subject(s) - differential centrifugation , size exclusion chromatography , chemistry , isoelectric point , isoelectric focusing , chromatography , intermolecular force , dissociation (chemistry) , protein–protein interaction , fractionation , biophysics , biochemistry , molecule , biology , organic chemistry , enzyme
This review summarizes theory, experimental procedures and applications of current research methodology in each stage of protein purification from the disruption of seed structures and isolation of protein bodies to cleavage of intermolecular bonds and the purification of individual proteins. Methods for characterizing proteins are discussed in terms of their utility in monitoring protein isolations, disclosing structural features and predicting protein behavior. Specific topics include: the macrostructure of seeds, scanning electron microscopy, particle fractionation by density gradient centrifugation, filtration, association‐dissociation of seed proteins, disulfide bonds and other forms of intermolecular bonding, high pressure gel filtration, immunospecific adsorbents or precipitating agents, immunochemical techniques, isoelectric focusing, physical and chemical methods for characterizing proteins, differential thermal analysis and the determination of polypeptide sequences.