Premium
Enzymatic enrichment of C 20 cis ‐5 polyunsaturated fatty acids from Biota orientalis seed oil
Author(s) -
Lie Ken Jie Marcel S. F.,
Rahmatullah M. S. K. Syed
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02638907
Subject(s) - lipase , polyunsaturated fatty acid , chemistry , fatty acid , hydrolysis , triacylglycerol lipase , chromatography , biochemistry , enzyme
Enrichment of cis ‐5 polyunsaturated fatty acids [20:3(5 c ,11 c ,14 c ), 4.3% and 20:4(5 c ,11 c ,14 c ,17 c ), 11.3%] from Biota orientalis seed oil was carried out by lipase‐catalyzed selective esterification and hydrolysis reactions. Lipases from Rhizomucor miehei (Lipozyme), Candida cylindracea and porcine pancreas were used. Lipozyme‐catalyzed esterification of Biota fatty acids with n ‐butanol in n ‐hexane allowed 20:3 and 20:4 (as fatty acids) to be enriched to a maximum level of 52.9%, and in the presence of C. cylindracea lipase 61.5% enrichment was achieved. Esterification with pancreatic lipase was poor with low levels of enrichment of 20:3 and 20:4 (22%). A multigram scale esterification of the free fatty acids from Biota seed oil by repeated treatment of the isolated fatty acid fraction with n ‐butanol in n ‐hexane in the presence of C. cylindracea lipase furnished an enrichment yield of 72.5% of a mixture of 20:3 and 20:4 fatty acids. Urea fractionation of the free fatty acids of Biota oil gave an initial enriched fraction of 20:3 (9.5%) and 20:4 (25.2%) which, upon treatment with C. cylindracea lipase in n ‐butanol and n ‐hexane, gave an enriched fraction of 85.3% of 20:3 and 20:4 fatty acids. Partial hydrolysis of the triglycerides of Biota oil by C. cylindracea lipase in potassium phosphate buffer at 25°C resulted in a 2.8‐fold enrichment of cis ‐5 polyunsaturated fatty acids (40.8% of 20:3 and 20:4) as contained in the unhydrolyzed acylglycerol fractions.