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Some properties of the lipase present in germinating rapeseed
Author(s) -
Wetter L. R.
Publication year - 1957
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02638019
Subject(s) - tributyrin , rapeseed , germination , lipase , chemistry , food science , substrate (aquarium) , glutathione , enzyme , triacylglycerol lipase , biochemistry , chromatography , horticulture , biology , ecology
Summary The lipase content of rapeseed germinated in light increases by 100‐fold over that of the dormant seed. The increase was much less in seeds germinated in the absence of light. A zero‐order reaction rate for the enzyme was observed when tributyrin was employed as the substrate. Optimum activity occurred at pH 8.5. The Michaelis‐Menton was calculated to be 4.06×10 −4 M, which is close to values reported for other lipases. While most substances tested had no effect on fat‐splitting activity, NaCN, NaN 3 , and glutathione behaved as activators.