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A method for the determination of the water‐insoluble combined lactic acid content of shortenings containing lactylated emulsifiers
Author(s) -
Fett Helen M.
Publication year - 1961
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637980
Subject(s) - acetaldehyde , chemistry , lactic acid , saponification , chromatography , sulfuric acid , glycerol , organic chemistry , ethanol , biology , bacteria , genetics
Shortenings containing glycerol lactopalmitates and glycerol lactostearates are analyzed for water‐insoluble combined lactic acid content using a procedure adapted from that of Barker and Summerson (1). Water‐soluble constituents are extracted from a chloroform solution of the shortening. The water‐washed shortening is saponified and then acidified to release the lactic acid, which is degraded to acetaldehyde by heating with concentrated sulfuric acid. The acetaldehyde is reacted with p ‐phenyl phenol is concentrated acid solution to produce a purple colored reaction product. The intensity of the color is proportional to the concentration of the acetaldehyde. Absorption is read at 570 mॖ using a lithium lactate solution as a standard. The method has been applied to the analysis of shortenings containing from 0.70 to 1.10% water‐insoluble combined lactic acid.