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Separation of petroselinic ( cis ‐6 18:1) and oleic ( cis ‐9 18:1) acids by gas‐liquid chromatography of their isopropyl esters
Author(s) -
Wolff Robert L.,
Vandamme Frederic F.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637686
Subject(s) - oleic acid , isopropyl , chemistry , chromatography , isopropyl myristate , gas chromatography , organic chemistry , methyl oleate , structural isomer , derivative (finance) , biochemistry , catalysis , financial economics , economics
Petroselinic ( cis ‐6 18:1) and oleic ( cis ‐9 18:1) acids that occur together in Umbelliferae seeds can be resolved by gasliquid chromatography (GLC) of their methyl or isopropyl esters on a 50 m × 0.25 mm fused‐silica capillary column coated with a 100% cyanopropyl polysiloxane stationary phase (CP Sil 88). The use of isopropyl esters instead of methyl esters increases the difference between equivalent chainlengths from 0.06 carbon unit up to 0.08. This is sufficient to obtain an almost base‐line resolution between the two components. cis ‐Vaccenic acid is completely separated from oleic acid in both derivative forms. GLC of fatty acid isopropyl esters on an appropriate capillary column thus appears to be the simplest means to simultaneously and accurately quantitate petroselinic, oleic and cis ‐vaccenic acids.