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Adsorption analysis of lipids. II. The fractionation of soybean oil and derived ethyl esters
Author(s) -
Reinbold Catherine L.,
Dutton Herbert J.
Publication year - 1948
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637645
Subject(s) - fractionation , glyceride , adsorption , iodine value , chemistry , iodine , soybean oil , chromatography , fraction (chemistry) , organic chemistry , fatty acid , biochemistry
Summary A rapid method of fractionating soybean glycerides and their ethyl esters by adsorption analysis on an aluminum oxide column is described. It compared favorably with other methods of fractionation. In the adsorption analysis of crude soybean oil, the iodine values of fractions ranged from 104 to 173, a spread of 69 units. However, under other conditions of adsorption, iodine values as high as 200 were obtained. In the analysis of ethyl esters of soybean oil, iodine values ranged from 61 to 185, a spread of 124 units. The fat acid composition of each fraction was calculated from spectrophotometric and iodine value determinations. Recoveries from the columns were 82.4% in the case of the glycerides, and 90.0% in the case of the esters.