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Hydrolysis of corn oil by lipase from Helminthosporium maydis race T
Author(s) -
Bennett G. A.,
Freer S.,
Shotwell O. L.
Publication year - 1976
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637390
Subject(s) - monoglyceride , lipase , chemistry , food science , hydrolysis , fatty acid , sucrose , extraction (chemistry) , corn oil , diglyceride , chromatography , thin layer chromatography , triglyceride , mycelium , biology , enzyme , biochemistry , botany , cholesterol
Corn oil was used as the sole carbon source for growth of Helminthosporium maydis Race T (NRRL 5128) in shaken flasks at 28 C. Oil recovered by hexane extraction from culture medium and mycelia at 2‐day intervals was analyzed by thin layer chromatography and gas chromatography. Triglyceride content of the oil was reduced, whereas free fatty acid, monoglyceride, and diglyceride contents increased as a result of Helminthosporium maydis growth. Free sterol and steryl ester contents were unaffected. Lipase production was demonstrated by Helminthosporium maydis cells grown on corn oil, but the enzyme was not detected in cells grown on sucrose.