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Rapid densitometric determination of triglyceride groups by argentation thin layer chromatography
Author(s) -
Chobanov D.,
Tarandjiska R.,
Chobanova R.
Publication year - 1976
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637389
Subject(s) - triglyceride , degree of unsaturation , sunflower oil , thin layer chromatography , chromatography , charring , chemistry , densitometry , tallow , food science , organic chemistry , cholesterol , biochemistry , physics , quantum mechanics
A method has been developed for the quantitative thin layer chromatographic determination of the triglyceride groups, differing in unsaturation, based on densitometry of the charred bands. Complete separation was achieved by continuous development in an open glass jar. The need for correction coefficients was avoided through addition of bromine to the double bonds prior to charring in the presence of sulphuryl chloride. The accuracy and precision of the method were evaluated on a standard mixture and on sunflower oil, olive oil, lard, cocoa butter, and beef tallow. The method is applicable for triglyceride group analysis of the most common fats and oils which contain saturated, monoene, and diene acids.