Premium
The simultaneous determination of both the quantity and the fatty acid composition of the triglycerides in three to ten microliters of plasma
Author(s) -
Bowers Cyril Y.,
Hamilton James G.,
Muldrey James E.,
Miyamasu Walter T.,
Reynolds George Ann,
Schally Andrew V.
Publication year - 1966
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02637267
Subject(s) - triglyceride , chemistry , chromatography , silicic acid , fatty acid , composition (language) , fraction (chemistry) , elution , silica gel , gas chromatography , biochemistry , organic chemistry , cholesterol , linguistics , philosophy
An integrated system using chromatography on silica gel‐impregnated glass paper, an internal standard and gas‐liquid chromatography (GLC) makes possible the isolation of triglycerides, the determination of triglyceride, fatty acid composition, and the quantitative determination of triglyceride. Recoveries are good and values for total triglyceride obtained by this system compare favorably with values obtained by application of the hydroxamate colorimetric method to the triglyceride eluate from silicic acid columns. The fatty acid composition of the cholesteryl ester (CE) fraction is obtainable at the same time by GLC of methyl esters prepared from the CE fractions which also appears as a distinct spot on these chromatograms. Also, a densitometric method is described for triglyceride determination by photometry of the charred spots obtained after chromatography on the gel‐paper.