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The effect of relative concentrations on the efficiency of separation of polar and nonpolar lipids by alumina column chromatography
Author(s) -
Sims R. P. A.,
Mes J. C.
Publication year - 1961
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636763
Subject(s) - silicic acid , polar , chemistry , solvent , elution , chromatography , phosphorus , chemical polarity , methanol , organic chemistry , molecule , physics , astronomy
Columns were packed with aluminas from various sources and of low and high adsorptive power. Polar and nonpolar lipids were eluted from them with strong and weak solvents, and the fractions were tested for phosphorus, nitrogen, sterols, and triglycerides. The conditions used by the 1956 Technical Committee of the N.S.P.A. for the chromatography of linseed oil gave the most complete separation of polar from nonpolar lipids when the former were present in low concentration. When the lipid phosphorus load approached 0.4 mg./gm. alumina however, none of the alumina systems tried was satisfactory although silicic acid columns resolved the mixtures completely. Gamma alumina had greater resolving power than alpha alumina monohydrate. Brockmann Grade I alumina with a strong solvent system gave better speration than weaker aluminas with weaker solvent systems.