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Comparison of methods for determining fatty acid oxidation produced by ultraviolet irradiation
Author(s) -
Kenaston Carolyn B.,
Wilbur Karl M.,
Ottolenghi Athos,
Bernheim Frederick
Publication year - 1955
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636476
Subject(s) - linolenate , chemistry , thiobarbituric acid , peroxide , linolenic acid , linoleic acid , ultraviolet irradiation , fatty acid , ultraviolet , organic chemistry , chromatography , irradiation , lipid peroxidation , antioxidant , materials science , physics , nuclear physics , optoelectronics
Summary The thiobarbituric acid (TBA) reaction for fatty acid oxidation has been compared with Lundberg and Chipault's method for peroxides, the Kreis test for aldehydes, and with the degree of conjugation, using fatty acid esters exposed to ultraviolet light for various periods. The TBA test paralleled the other methods for methyl linolenate and methyl linoleate but was essentially negative for methyl oleate oxidation. The sensitivity of the TBA test for linolenate was 30–80 times that for linoleate at the same peroxide values. The TBA test appears to be a reliable method of estimating the oxidation products of linolenic and linoleic acids in tissues and other biological material.

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