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Determination of individual tocopherols by derivative spectrophotometry
Author(s) -
Bukovits Gabriel J.,
Lezerovich Alberto
Publication year - 1987
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636386
Subject(s) - derivative (finance) , spectrophotometry , chromatography , second derivative , chemistry , analytical chemistry (journal) , mathematics , mathematical analysis , financial economics , economics
The second order derivative UV spectra of α‐, β‐, γ‐ and σ‐tocopherols showed small differences that allowed development of a derivative spectrophotometric method for determining individual tocopherols in a mixture. The procedure consists of measuring the second derivative spectrum of the sample at four specified wavelengths and calculating each concentration by means of a method for multicomponent analysis that uses mixtures as standards. Vegetable oils and other fatty products usually require a previous clean‐up process (under study) in order to remove interfering substances.