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Quantitative determination of BHT in soap products by capillary gas chromatography
Author(s) -
Goldstein M. M.,
Molever K.,
Lok W. P.
Publication year - 1982
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636328
Subject(s) - chemistry , derivatization , chromatography , bstfa , gas chromatography , detection limit , silylation , flame ionization detector , standard addition , organic chemistry , high performance liquid chromatography , catalysis
A simple and rapid capillary gas chromatography (GC) method is described for the quantitative determination of 2,6‐di‐ tert ‐butyl‐4‐methylphenol (BHT) antioxidant in soap bars, fatty acids, and related intermediates. The procedure involves blending the sample with dimethylformamide in the presence of 2,4‐di‐ tert ‐butylphenol (DTBP) internal standard, filtering the mixture, silylating an aliquot with BSTFA (bis‐trimethylsilyltrifluoroacetamide) and quantifying by capillary GC using flame ionization detection. The silyl derivatization and nonpolar capillary column (12 m, methyl silicone, fused silica) provided resolution of BHT from certain fragrance component interferences. The method has a detection limit of approximately 10 ppm. Soaps fortified with BHT showed recoveries of 97.1±3.7% at the 200 ppm level and 92.3 ± 2.2% when spiked at the 75 ppm level. The effect of bar soap storage time on BHT content is also demonstrated.