Premium
Distribution of ammonia‐related aflatoxin reaction products in cottonseed meal
Author(s) -
Park Douglas L.,
Lee Louise,
Koltun S. A.
Publication year - 1984
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636223
Subject(s) - aflatoxin , cottonseed meal , chemistry , residue (chemistry) , meal , ammonia , chromatography , methanol , chloride , food science , soybean meal , biochemistry , organic chemistry , raw material
The fate of aflatoxin during ammoniation of contaminated cottonseed meal was studied under conditions approximating those approved for commercial ammoniation of nonaflatoxin‐contaminated meal. Uniformly ring‐labeled 14 C‐aflatoxin B 1 was added to 27.7 kg meal (14% moisture) that contained ca. 4000 µg naturally incurred aflatoxin B 1 /kg. Distribution of the radiolabeled compound was used to trace the modification of aflatoxin B 1 after treatment with 4% ammonia at 40 psi, 100 C for 30 min. This treatment reduced the chemically detected aflatoxin B 1 to less than 4 µg/kg. In control nonammoniated meals, 90% of the radiolabeled material was accounted for in the methylene chloride extract. Duplicate 2‐kg samples of the ammoniated meal were fractionated and the distribution of radioactivity was determined. Ca. 86% of the radioactivity was detected in the meal after initial air‐drying. Ca. 25% of the added radioactivity was extracted from the air‐dried meal with methylene chloride and another ca. 5% was extracted from this residue with methanol. Weak acid released 3% of the added radioactivity from the residue after methanol extraction, bicarbonate released 1% and Pronase digestion, including methylene chloride extraction of the residue, accounted for nearly 19% of the total added radioactivity. Only 37% of the added radioactivity remained in the meal matrix following solvent extractions and chemical and enzymic treatments.