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Supercritical fluid fractionation of fish oil esters using incremental pressure programming and a temperature gradient
Author(s) -
Nilsson William B.,
Gauglitz Erich J.,
Hudson Joanne K.
Publication year - 1989
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636184
Subject(s) - fractionation , supercritical fluid , fish oil , yield (engineering) , chromatography , temperature gradient , chemistry , fish <actinopterygii> , analytical chemistry (journal) , materials science , organic chemistry , fishery , composite material , physics , quantum mechanics , biology
Abstract Incremental pressure programming was demonstrated to be an effective technique for increasing the yield of 90% pure all cis ‐5,8,11,14,17‐ethyl eicosapentaenoate (EPA) in the fractionation of urea‐crystallized fish oil ethyl esters using supercritical fluid CO 2 . The fractionations, which also produced high purity all cis ‐4,7,10,13,16,19‐ethyl docosahexaenoate (DHA), were performed using a column temperature gradient. In initial experiments, the maximum temperature of the superimposed gradient was 80°C, and processing pressures ranged from 1900–2200 psi. By reduction of processing pressures, comparable yields of EPA were obtained from fractionations in which the maximum temperatures in the gradient were 70°C and 60°C.