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Growth and eicosapentaenoic acid production by Phaeodactylum tricornutum in batch and continuous culture systems
Author(s) -
Yongmanltchal Wichien,
Ward Owen P.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636113
Subject(s) - phaeodactylum tricornutum , eicosapentaenoic acid , dilution , chemostat , food science , chemistry , chromatography , zoology , biomass (ecology) , fatty acid , volume (thermodynamics) , biology , biochemistry , botany , polyunsaturated fatty acid , algae , physics , genetics , quantum mechanics , bacteria , thermodynamics , agronomy
Maximum specific growth rate (μ max ) of Phaeodactylum tricornutum increased with increasing culture reactor surface‐to‐volume ratio. Values for μ max of 0.647, 0.377 and 0.339 day −1 were observed for the 75‐mL tube, 5.6‐L tank and the 16‐L tank, respectively. Higher biomass was achieved in the 75‐mL batch culture tube under continuous light as compared with light cycle conditions. Palmitic acid, palmitoleic acid and eicosapentaenoic acid (EPA) accounted for over 60% of total fatty acids in the batch tube culture, with EPA content increasing to a maximum after three days. In chemostat cultures, run at dilution rates of 0.15 day −1 (0.45 of μ max ) and 0.3 day −1 (0.9 of μ max ), cell concentration reached a steady state of 2.18 and 0.7 g/L, respectively, while contents of EPA per liter of culture at steady state were 100.9 and 82.5 mg/L, respectively. At both dilution rates, EPA content of total fatty acids was the same (35.0–35.2%). At a dilution rate of 0.3 day −1 , the continuous culture system manifested productivities of 0.51 g/L/d and 25.1 mg/L/d for biomass and EPA, respectively.