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Study of neutral lipids of Lupinus mutabilis meal and isolates
Author(s) -
Millan Francisco,
Alaiz Manuel,
HernandezPinzon Inmaculada,
SanchezVioque Raul,
Bautista Juan
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636091
Subject(s) - chemistry , isoelectric point , meal , food science , chromatography , lupinus angustifolius , composition (language) , biochemistry , botany , biology , linguistics , philosophy , enzyme
Abstract Two types of protein isolates have been obtained from defatted Lupinus mutabilis meal. The isolates, MA and MB, were obtained by alkaline extraction with 0.2% NaOH and 0.25% sodium bisulfite, respectively, followed by precipitation at the isoelectric point (pH 4.8). Total associated lipids were extracted with 86% ethanol. Neutral lipids were separated in a Florisil column. The lipids in the isolates were similar to those found in the original meal. The following types of compounds were separated, identified, and quantitated: hydrocarbons, waxes, methyl esters, triacylglycerols, free fatty acids, diacylglycerols, and free sterols.