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Application of water mimics on preparation of eicosapentaenoic and docosahexaenoic acids containing glycerolipids
Author(s) -
Hosokawa Masashi,
Takahashi Koretaro,
Miyazaki Noriko,
Okamura Keiji,
Hatano Mutsuo
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636082
Subject(s) - transesterification , docosahexaenoic acid , eicosapentaenoic acid , chemistry , phosphatidylcholine , hydrolysis , ethylene glycol , lipase , organic chemistry , fish oil , glycerol , methanol , chromatography , fatty acid , polyunsaturated fatty acid , phospholipid , biochemistry , enzyme , fish <actinopterygii> , biology , membrane , fishery
To obtain enhanced incorporation of highly unsaturated fatty acids and recovery of glycerolipid products, organic solvents with high dielectric constants (water mimics) were substituted for part of the essential water for lipase activation to study their effect on acidolysis and transesterification. In acidolysis/transesterification of fish oil triglycerides and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), Lipozyme IM‐60 with ethylene glycol as a water mimic enhanced the incorporation of EPA and suppressed the hydrolysis of synthesized glycerolipid. On the other hand, transesterification between soy phosphatidylcholine and EPA was enhanced by a water and propylene glycol combination. In a nonaqueous medium that contained appropriate amounts of water and organic solvents (water mimics), Lipozyme IM‐60 increased transesterification of EPA into soy phosphatidylcholine. Simultaneously, the recovered glycerolipid products showed decreased hydrolysis of newly synthesized EPA‐ and DHA‐containing glycerolipids.