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Production of 15‐, 16‐ and 17‐hydroxy‐9‐octadecenoic acids by bioconversion of oleic acid with Bacillus pumilus
Author(s) -
Lanser A. C.,
Plattner R. D.,
Bagby M. O.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636069
Subject(s) - bioconversion , oleic acid , chemistry , bacillus pumilus , chromatography , gas chromatography , mass spectrometry , organic chemistry , gas chromatography–mass spectrometry , biochemistry , biology , bacteria , fermentation , genetics
Several Bacillus strains were tested for their ability to hydroxylate oleic acid. Two strains—BD‐174 and BD‐226—converted oleic acid into a trio of hydroxy‐octadecenoic acids. Bioconversion in 72–120 hr produced 5–11% of hydroxy acids relative to oleic acid as measured by gas chromatography. These acids were identified as the 15‐, 16‐ and 17‐hydroxy‐9‐octadecenoic acids by gas chromatography‐mass spectrometry of trimethyl silyl derivatives of the product acids and their hydrogenated counterparts.