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Formation of trierucoylglycerol (trierucin) from 1,2‐Dierucoylglycerol by a homogenate of microspore‐derived embryos of Brassica napus L
Author(s) -
Taylor David C.,
Weber Nikolaus,
Hogge Lawrence R.,
Underhill Edward W.,
Pomeroy M. Keith
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636067
Subject(s) - microspore , erucic acid , brassica , acyltransferase , stereospecificity , biochemistry , endogeny , embryo , chemistry , diacylglycerol kinase , rapeseed , biology , enzyme , botany , stamen , microbiology and biotechnology , pollen , protein kinase c , catalysis
Homogenates of microspore‐derived embryos of rape ( Brassica napus L.) incubated with [1‐ 14 C]erucoyl‐CoA and 1,2‐dierucoylglycerol are able to assemble trierucoyl‐glycerol (trierucin). In addition, radioactive triacylglycerols are formed by transferring [1‐ 14 C]‐erucoyl moieties to endogenous lipid precursors. Stereospecific analysis of radioactive triacylglycerols revealed that labeled erucoyl moieties had been incorporated exclusively into the sn ‐1,3 positions with more than 95% of radioactivity in the sn ‐3 position. No incorporation of labeled erucic acid into the sn ‐2 position has been observed. All data agree with the involvement of 1,2‐diacylglycerol acyltransferase (E.C. 2.3.1.20), which utilized 1,2‐dierucoylglycerol as well as endogenous 1,2‐diacylglycerols as acceptors of erucoyl moieties. This result is of particular interest for the genetic modification of rape and other Cruciferae for producing trierucin in their seed oils.