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Separation and quantitation of hydroxy and epoxy fatty acid by high‐performance liquid chromatography with an evaporative light‐scattering detector
Author(s) -
Gérard Hervé C.,
Moreau Robert A.,
Fett William F.,
Osman Stanley F.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02636054
Subject(s) - chromatography detector , chromatography , high performance liquid chromatography , chemistry , detector , detection limit , hexane , epoxy , light scattering , fatty acid , analytical chemistry (journal) , scattering , organic chemistry , optics , physics
A new high‐performance liquid chromatography technique with an evaporative light‐scattering detector (ELSD) has been developed for the separation and quantitative analysis of hydroxy and epoxy fatty acids. This method employs a gradual binary gradient (hexane/isopropanol) and ELSD detection. The minimum limit of detection is about 1 µg and ratio of mass to signal is essentially linear in the range of 10 to 200 µg. This high‐performance liquid chromatography (HPLC) technique is able to separate various positional isomers of mono‐hydroxy and dihydroxy fatty acids and can also discriminate between monohydroxy, epoxy, epoxyhydroxy, dihydroxy and trihydroxy fatty acids.